Upcoming: 4th Tofo Advanced Study Week, Sep. 2019
Tofo Beach

Enjoy excellent science in a beautiful African setting

Scientific Program
      Tofo Logo 2019

Sunday, September 01, 2019

17:00 – 21:00 Welcome reception at Top-of-the-Dune Guesthouse
                       Registration of conference attendees

Monday, September 02, 2019

08:00            Registration desk open
09:00            Opening ceremony
10:00            Coffee/tea break
10:30            Rolf Hilgenfeld: A few housekeeping remarks

Session 1 – Natural and man-made disasters and arbovirus disease
Chair: Belayneh Getachew (Debre Zeit, Ethiopia)

10:45 – 11:00 Eduardo Samo Gudo (Maputo, Mozambique)
                       Introduction and overview

11:05 – 11:20 Bibiana Melchior (Maputo, Mozambique)
Evaluation of the arbovirus rapid test during a cyclone emergency in Mozambique

11:25 – 11:40 Osvaldo Inlamea (Maputo, Mozambique)
Evidence of arboviruses and leptospirosis among outpatients with acute febrile illness and malaria negative results in areas affected by cyclones Idai and Kenneth in Mozambique

11:45 – 12:00 Ulrich Vickos (Bangui, Central African Republic)
Viral hepatitis E, yellow fever, monkeypox, rabies, measles, poliomyelitis... from September 2018 to June 2019. What will be the next epidemic?

12:05 - 12:25 Keynote: Sina Bavari (Frederick, MD, USA)
Emerging viral diseases: viral replication to vaccines and therapeutics

12:30 – 13:30        Lunch

Session 2 – Vaccine Development I
Chair: Stephen Streatfield (Newark, DE, USA)

13:30 – 13:50 Vijaya Satchidanandam (Bangalore, India)
Role of viral glycoproteins in attenuation of JEV vaccine SA14-14-2

13:55 – 14:15 Hui Chen (Beijing, China)
Vaccination with a single consensus envelope protein ectodomain sequence administered in a heterologous regimen induces tetravalent immune response and protection against Dengue viruses in mice

14:20 – 14:40 Amelie Schoenenwald (Vienna, Austria)
Developing scFv antibodies against Usutu virus envelope protein domain III using phage display

Session 3 – Epidemiology I: Flaviviruses and alphaviruses
Chair: Eduardo Samo Gudo (Maputo, Mozambique)

14:45 - 15:00 Lameck Agasa (Nairobi, Kenya)
Spatial statistical analysis and modeling of incidences of Chikungunya virus in Kenya

15:05 – 15:20 Plácida Maholela (Maputo, Mozambique)
Retrospective investigation of IgM antibodies against Zika virus in serum from febrile patients in Mozambique, 2009-2015

15:25 – 15:40 Pedro Martínez (Luanda, Angola)
Haemorrhagic fever syndromes recorded during the arbovirus surveillance in Angola, 2018

15:45 – 16:05 Coffee/tea break

16:05 – 16:20 Felicity Burt (Bloemfontein, South Africa)
Sindbis antibody seroprevalence in select populations in the central plateau of South Africa

16:25 – 16:40 Matefo Litabe (Bloemfontein, South Africa)
In-vitro immune responses to Sindbis virus

16:45 – 17:00 Sadia Ali Pereira (Maputo, Mozambique)
Emerging and re-emerging arbovirus in Mozambique: implication in acute febrile illness management

17:05 – 17:20 Matthew Valentine (St. Kitts, West Indies)
Investigating sylvatic cycles of Dengue, Chikungunya and Zika viruses on St. Kitts

17:25 – 17:40 Asabe Dzikwi-Emennaa (Jos, Nigeria)
Serological evidence of Rift Valley Fever infection among camels in North-Western Nigeria

Session 4 - Novel diagnostic approaches
Chair: Kerstin Falk (Stockholm, Sweden)

17:45 – 18:00 Severino Jefferson Ribeiro da Silva (Recife, Brazil)
Simple, fast and sensitive Zika virus assay using reverse transcription loop-mediated isothermal amplification

18:05 – 18:20 Lawrence Ofosu-Appiah (Accra, Ghana)
An evaluation of the performance characteristics of an immunochromatographic rapid diagnostic test kit for the detection of yellow fever virus IgM in Ghana

18:25 – 18:40 Tshephang Iris Kabelo (Palapye, Botswana)
Assessment of the sensitivity of primary cells and cell lines to the Southern African Territories serotypes in the diagnosis of Foot-and-Mouth Disease virus

20:00 – 21:00 TBC: Talk on marine wildlife in Tofo (Casa Barry)

Tuesday, September 03, 2019

08:30 – 12:00 Guided tour of Inhambane town
08:30 – 10:30 Ocean safari

12:30 – 13:30 Traditional matapa lunch in an African village

14:00 – 14:20 Focus on coronaviruses: Zhengli Shi (Wuhan, China)
Risk assessment of MERS-cluster coronaviruses in China

Session 5 – Molecular biology of flaviviruses
Chair: Cheng-Feng Qin (Beijing, China)

14:25 – 14:40 Ankur Kumar (Mandi, India)
Functional implication of intrinsically disordered proteins: Zika virus protease complex as a model system

14:55 – 15:10 João A. R. G. Barbosa (Brasilia, Brazil)
Structural analysis of proteases NS3 of flavivirus Zika and Dengue, and nsP2 of alphavirus Chikungunya and Mayaro by in silico and in vitro tools

15:15 – 15:30 Rajanish Giri (Mandi, India)
Zika virus helicase: from biophysics towards drug discovery of a moving target

15:35 – 15:55        Coffee/tea break

Session 6 – Antiviral drug discovery
Chair: Rajanish Giri (Mandi, India)

15:55 – 16:10 Mila Leuthold (Heidelberg, Germany)
DENV2proHeLa: a rapid, cell-based screening assay to study DENV serotype 2 protease inhibitors

16:15 – 16:30 Frank Kozielski (London, UK)
Discovery of novel fragments hits targeting Dengue and Zika virus non-structural protein 5 using x-ray crystallography-based fragment screening

16:35 – 16:50 Tomas Bergström (Gothenburg, Sweden)
Search for antiviral compounds against Tick-borne Encephalitis virus – an emerging CNS infection in Eurasia

16:55 – 17:10 Elijah Eze Ajaegbu (Enugu, Nigeria)*
Active principles from Spondias mombin leaf: preventive measures for Zika virus outbreak in Africa

17:15 – 17:30 Jörg Rademann (Berlin, Germany)
Fragment-based reversible covalent inhibitors of enterovirus 3C proteases

17:35 – 17:50 Jackson Thomas Mollel (Gothenburg, Sweden & Dar-es-Salaam, Tanzania)
Anti-respiratory syncytial virus activity of medicinal plants from Kagera Region in Tanzania

17:55 – 18:10 Vanessa Monteil (Stockholm, Sweden)
Three-dimensional human hepatocyte spheroids as a model system for antiviral screening

18:15 – 18:30 Aleksandra Pettke (Stockholm, Sweden)
When antiviral is not enough – exploring a new strategy in the fight against Zika virus

Wednesday, September 04, 2019

Session 7 - Viral pathogenesis
Chair: Scott Pegan (Athens, GA, USA)

09:00 – 09:20 Cheng-Feng Qin (Beijing, China)
Zika virus neurotropism: the bad and the good

09:25 – 09:45 Peigang Wang (Beijing, China)
Zika virus infection in hypothalamus causes multi-hormone deficiencies and leads irreversible growth delay and memory impairment in mice

09:50 – 10:10 Jing An (Beijing, China)
Axl deficiency increases the mortality of Japanese Encephalitis via enhancing IL-1α and blood brain barrier permeability

10:15 – 10:35 Suresh Mahalingam (Gold Coast, Australia)
Understanding how arthritogenic alphaviruses cause arthritis

10:40 – 11:00 Allen Z. Wu (Nanjing, China)
Pathogenesis of a novel phlebovirus is associated with defective B cell immunity and skewed macrophage differentiation

11:05 – 11:25        Coffee/tea break

Session 8 - Molecular biology of negative-strand RNA viruses
Chair: Thomas Strecker (Marburg, Germany)

11:25 – 11:40 Sweety Samal (Faridabad, India)
Development of native-like Influenza matrix 2 ectodomain soluble protein as immunogen candidate and other applications

11:45 – 12:00 Luca Zinzula (Munich, Germany)
A tripartite split-GFP based fluorescence complementation assay to study the Ebola and Marburg virus VP35 oligomerization domain

12:05 – 12:20 Roland Hartmann (Marburg, Germany)
“Rule of 6” governs transcription initiation in the genomic 3’ – leader of Ebola virus

12:25 – 12:40 Scott Pegan (Athens, GA, USA)
CCHFV host immunity suppression and creation, a story of a viral OUT protease and viral replicon particles

12:45 - 13:00 Yi Shi (Beijing, China)
Structural insight into regulation of RNA synthesis by influenza polymerase

13:05 – 13:50     Lunch

Session 9 - Vaccine development II
Chair: Vijaya Satchidanandam (Bangalore, India)

13:50 – 14:10 Djibril Wade (Dakar, Senegal)
Safety and immunogenicity of a heterologous prime-boost Ebola virus vaccine regimen – ChAd3-EBO-Z followed by MVA-EBO-Z in healthy adults in Senegal: EBL06 clinical trial

14:15 – 14:35 Thomas Strecker (Marburg, Germany)
Virus-like particles expressing native-like Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with neutralizing activity

14:40 – 15:00 Stephen Streatfield (Newark, DE, USA)
Plant-based biomanufacturing for subunit vaccines and antibodies to combat emerging and re-emerging infectious diseases

Session 10 - Epidemioloy II
Chair: Felicity Burt (Bloemfontein, South Africa)

15:05 – 15:20 Jia Kangbai (Njala, Sierra Leone)
Epidemiological characteristics, clinical manifestations, and treatment outcomes of 139 paediatric Ebola patients treated at a Sierra Leone Ebola treatment center

15:25 – 15:40 Veerle Msimang (Johannesburg, South Africa)
Seroprevalence of Crimean-Congo Haemorrhagic Fever in cattle and humans in South Africa, 2017: a cross-sectional study

15:45 – 16:00 John Oludele (Maputo, Mozambique)
Seroepidemiology of CHFV, Rickettsia, Brucella, and Leptospira among pastoral communities in Sofala Province, Mozambique, 2018

16:00 – 16:10 Group photograph
16:10 – 16:30 Coffee/tea break

16:35 – 16:50 Pedro Martinez (Luanda, Angola)
Influenza A (H1N1) pdm09 detection in an influenza-like illness outbreak in Luanda, Angola, 2019

Session 11: Vector biology and control
Chair: Sadia Ali Pereira (Maputo, Mozambique)

16:55 – 17:15 Gong Cheng (Bejing, China)
A gut commensal bacterium promotes mosquito permissiveness to arboviruses

17:20 – 17:40 Tonya Colpitts (Boston, MA, USA)
 Arbovirus host factors: discovery and targeting

17:45 – 18:00 Alexander Gold (Boston, MA, USA)
The role of exosomes in human to mosquito transmission of Dengue virus

18:05 – 18:25 Judith Wong (Singapore)
Singapore's mosquito-borne disease surveillance and vector control programme

18:35 – 18:45 Closing Remarks
Eduardo Samo Gudo (Maputo, Mozambique)
Rolf Hilgenfeld (Lübeck, Germany)

19:30 – 23:00     Conference banquet (Hotel Tofo Mar)

Thursday, September 05, 2019

07:30 – 9:30     2nd Ocean safari

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